２０１０	Presentation at American Chemical Society (ACS) Conference in Boston, New Jersey USA 【Title】 De-convolution of medicinal citrus products Shiming Li1, Yutaka Miyauchi2, Michiko Suzawa2, Terry Nash2, Nancy E. Rawson1, Chi-Tang Ho3 1)　WellGen, Inc., North Brunswick, NJ USA 2)　Miyauchi Citrus Research Center, Ltd., 610 Iwai, Yoshii-machi, Takasaki, Gunma, Japan 3)　Department of Food Science, Rutgers University, New Brunswick, NJ USA Introduction of Citrus Products on the market: Gold Lotion, Sytrinol <Medicinally used products> Sytrinol, KGK Synergizer, Canada 　▪ Components disclosed: Polymethoxyflavones (tangeretin, nobiletin, etc), Tocotrienols 　▪ Major claims: Recommended dosage 300 mg/day/person, lowering total cholesterol and LDL 　　cholesterol by up to 27%, compared to placebo, improving triglycerides up to 34%, 　　compared to placebo. <Gold Lotion> Biological activities 　▪ Anti-inflammation: iNOS, TNFα 　▪ Anti-carcinogenic: details follow 　▪ Anti-microbial: S. aurenus, S. griseus, P. digitatum, F. oxysporus, T. aentagrophytes, M. gypseum, 　　P. aeruginosa, V. parahacmolyticus, etc. 　▪ Hair growth stimulating agent 　▪ Skin whitening <Biological activity of citrus compounds> Flavonoids (hesperidin, etc.), PMFs (polymethoxyflavones), OH-PMFs (hydroxylated polymethoxyflavones) 　▪ Anti-inflammatory Activity of PMFs and OH-PMFs ▻ Down-regulation of multiple enzymes: COX-2, iNOS, myeloperoxidase, 　TNF-α MMP-1, MMP-3, MMP-9, PGE2, etc ▻ Suppression of gene expression: COX-2, iNOS, IL-1a, IL-1b, TNF-α and IL-6, AP-1, NF-kB, etc. ▻ Product inhibition: NO, O2-, PGE2 ▪ Anti-carcinogenic Property of PMFs and OH-PMFs ▻ Inhibition of tumor cell proliferation ▻ Inhibition of NO, O2-, PGE2 production and tumor promotion ▻ Induction of apoptosis 　▪ OPE (orange-peel extract): Cancer Prevention and Treatment 　▻ Inhibition of cellular growth without cell death 　　　　Patented by (1)KGK Synergize Inc., 2008; (2)Ho et al, Rutgers ▻ Increased apoptosis in ductal epithelial cells of mouse mammary gland 　 (Journal of Medi Food, 2007, 10, 11) ▻ Nobiletin against lung cancer induced by cigarette smoking ▻ Synergistic effect in H2O2-induced apoptosis in human neuroblastoma SH-SY5Y cells, produced 　 by the combination of tangeretin and 5-demethylnobiletin 　▪ Anti-atherosclerosis Activity 　▪ Apoptotic activity of PMF Metabolites 　▪ Other Applications ▻ Anti-fungal agents against Penicillin digitatum 　　　Viticultura Enologia Professional, 2006, 91-99 ▻ Anti-Obesity drug from PMFs and OH-PMFs 　▪ OH-PMF Bioactivity compared with PMFs 　▪ Introduction of PMF Content in Gold Lotion Extract
	<PacifiChem 2010, Honolulu, Hawaii, United States, Dec. 15-20, 2010> Shiming Li, Michiko Suzawa, Terry Nash, Nancy E. Rawson, Chi-Tang Ho. Health promoting use and composition analysis of commercial citrus peel products. Abstracts of Papers <240th ACS National Meeting, Boston, MA, United States, Aug. 22-26, 2010, MEDI-238> Shiming Li, Michiko Suzawa, Terry Nash, Nancy E. Rawson Chi-Tang Ho. De-convolution of medicinal citrus products. Abstracts of Papers