2010 Presentation at American Chemical Society (ACS)
Conference in Boston, New Jersey USA

De-convolution of medicinal citrus products
Shiming Li1, Yutaka Miyauchi2, Michiko Suzawa2, Terry Nash2, Nancy E. Rawson1, Chi-Tang Ho3

1) WellGen, Inc., North Brunswick, NJ USA
2) Miyauchi Citrus Research Center, Ltd., 610 Iwai, Yoshii-machi, Takasaki, Gunma, Japan
3) Department of Food Science, Rutgers University, New Brunswick, NJ USA

Introduction of Citrus Products on the market: Gold Lotion, Sytrinol

<Medicinally used products> Sytrinol, KGK Synergizer, Canada
 ▪ Components disclosed: Polymethoxyflavones (tangeretin, nobiletin, etc), Tocotrienols
 ▪ Major claims: Recommended dosage 300 mg/day/person, lowering total cholesterol and LDL
  cholesterol by up to 27%, compared to placebo, improving triglycerides up to 34%,
  compared to placebo.

<Gold Lotion> Biological activities
 ▪ Anti-inflammation: iNOS, TNFα
 ▪ Anti-carcinogenic: details follow
 ▪ Anti-microbial: S. aurenus, S. griseus, P. digitatum, F. oxysporus, T. aentagrophytes, M. gypseum,
  P. aeruginosa, V. parahacmolyticus, etc.
 ▪ Hair growth stimulating agent
 ▪ Skin whitening

<Biological activity of citrus compounds>
Flavonoids (hesperidin, etc.), PMFs (polymethoxyflavones), OH-PMFs (hydroxylated polymethoxyflavones)
 ▪ Anti-inflammatory Activity of PMFs and OH-PMFs

▻ Down-regulation of multiple enzymes: COX-2, iNOS, myeloperoxidase,
 TNF-α MMP-1, MMP-3, MMP-9, PGE2, etc

▻ Suppression of gene expression: COX-2, iNOS, IL-1a, IL-1b, TNF-α and IL-6, AP-1, NF-kB, etc.

▻ Product inhibition: NO, O2-, PGE2

▪ Anti-carcinogenic Property of PMFs and OH-PMFs
▻ Inhibition of tumor cell proliferation

▻ Inhibition of NO, O2-, PGE2 production and tumor promotion

▻ Induction of apoptosis

 ▪ OPE (orange-peel extract): Cancer Prevention and Treatment
 ▻ Inhibition of cellular growth without cell death
    Patented by (1)KGK Synergize Inc., 2008; (2)Ho et al, Rutgers

▻ Increased apoptosis in ductal epithelial cells of mouse mammary gland
  (Journal of Medi Food, 2007, 10, 11)

▻ Nobiletin against lung cancer induced by cigarette smoking

▻ Synergistic effect in H2O2-induced apoptosis in human neuroblastoma SH-SY5Y cells, produced
  by the combination of tangeretin and 5-demethylnobiletin

 ▪ Anti-atherosclerosis Activity
 ▪ Apoptotic activity of PMF Metabolites
 ▪ Other Applications

▻ Anti-fungal agents against Penicillin digitatum
   Viticultura Enologia Professional, 2006, 91-99

▻ Anti-Obesity drug from PMFs and OH-PMFs
 ▪ OH-PMF Bioactivity compared with PMFs
 ▪ Introduction of PMF Content in Gold Lotion Extract

<PacifiChem 2010, Honolulu, Hawaii, United States, Dec. 15-20, 2010>
Shiming Li, Michiko Suzawa, Terry Nash, Nancy E. Rawson, Chi-Tang Ho.

Health promoting use and composition analysis of commercial citrus peel products.
Abstracts of Papers

<240th ACS National Meeting, Boston, MA, United States, Aug. 22-26, 2010, MEDI-238>
Shiming Li, Michiko Suzawa, Terry Nash, Nancy E. Rawson Chi-Tang Ho.

De-convolution of medicinal citrus products.
Abstracts of Papers