2013年 International Conference & Exhibition　on Nutraceuticals and Functional Foods

１１月５日〜１１月９日 台北、台湾にて

リン博士による発表

複数の柑橘果皮を調合したエキスが免疫樹状細胞の機能を弱め、

アレルギー性接触過敏症を減少させる

＜要旨内容＞

柑橘フルーツの皮から調合されて出来た商品、ゴールド化粧水は、抗腫瘍、抗酸化や抗炎症作用などのような多くの薬理作用を持っている。今回の我々の研究ではLPSリポ多糖類で刺激させてマウスの骨髄から発生させた^＊樹状細胞発育（骨髄系樹状細胞）と機能に関してゴールドの免疫調節作用を調べた。結果、ゴールドは炎症前駆体サイトカインと^＊ケモカインの発現を量依存的に顕著に抑制した。更にゴールドでの処置は^＊MHCや共起刺激分子（アクセサリー細胞の膜結合性あるいは、分泌性産物でシグナル伝達が必要なもの）の発現を抑制。これは抗原を取り込む作用に影響を与えているとみなされる。また、LPS刺激により発生させた樹状細胞のアロ反応性T細胞活性化作用を減少させた。更に動物実験でのゴールドの経口投与では、2,4-ジニトロフルオロベンゼンによる接触過敏症（CHS）を低下させた。一貫して分子検査でもゴールドはLPS刺激により発生させた^＊MAPK-ERK分裂促進因子活性化蛋白質キナーゼ、p38リン酸化、核内転写因子NF-κB p65を減少させた。これらから今回の発見はゴールドの免疫薬理学に対する役割に新しい見識をもたらすものであり、樹状細胞介入による慢性の炎症性障害や自己免疫疾患症を治療できると考えられる。

＊樹状細胞：抗原提示細胞として機能する免疫細胞の一種であり哺乳類の免疫系の一部を担う。抗原提示細胞とは自分が取り込んだ抗原を他の免疫系の細胞に伝える役割を持つ。抗原を取り込むと樹状細胞は活性化され、脾臓などのリンパ器官に移動する。リンパ器官では取り込んだ抗原に特異的なT細胞やB細胞を活性化する。樹状細胞は発現している表面抗原分子により様々なサブユニットに分類される。）

＊ケモカイン：G蛋白質共役受容体を介してその作用を発現する塩基性蛋白質であり、サイトカインの一群。白血球などの郵送を引き起こし炎症の形成に関与。これまでに５０種類以上のケモカインが同定されている。

＊MHC：主要組織適合遺伝子複合体（免疫担当細胞間の認識機構・組織特異抗原や移植適合性をつかさどる細胞表面糖タンパクをコードする遺伝子領域で蜜に連鎖して一定の染色体領域に存在する。マウスではH-2、ヒトではHLA複合体よりなる。

リン博士は下記についても文献の用意をしているとの事です。

１） 肺癌に対するゴールドの抗腫瘍作用：１０％でステージ２への進行を止め、自家融解（細胞内の損傷を受けた細胞小器官の処理と分離）を起こす。

２） ゴールド経口投与による乾癬症における免疫薬理学作用

Formulated extract from multiple citrus peels impairs immune dendritic cells functions and attenuates allergic contact hypersentivity

Gold lotion (GL), a formulated product made from the peels of citrus fruits, has many pharmacological activities, such as anti-tumor, antioxidant, and anti-inflammatory activities. In this study, we examine the immunomodulatory effect of GL on lipopolysacchride (LPS) stimulated mouse bone marrow-derived DCs maturation and function. Our experimental results show that GL significantly inhibited the pro-inflammatory cytokines and chemokine expression in LPS-induced DCs in a dose dependent manner. In addition, GL treatment led to inhibit the expression of of MHC and costimulatory molecules, influence the ability to take up antigen and attenuated allo-T cell activating ability of LPS stimaultaed DCs. Furthermore, oral administration of GL attenuates the 2,4-Dinitro-1-fluorobenzene induced contact hypersensitivit (CHS) in vivo. Consequently, molecular studies showed that GL decrease LPS-induced MAPK-ERK, p38 phosphrylation and nuclear translocation of NF-κB p65. Taken together, these findings provide new insight into the immunopharmacological role of GL and used to treat DC mediated-chronic inflammatory disorders or autoimmune diseases.

Introduction of Researchers & Universities

Shiming Li^1,1a, Yi-Chin Lin², Chi-Tang Ho¹, Ping-Yi Lin³, Michiko Suzawa⁴, Hsin-Chieh Wang², Ching-Liang Chu⁵, Der-Yuan Chen^2,6, Chi-Chen Lin^*2,6,7,8,

¹Department of Food Science, Rutgers University, New Brunswick, NJ 08901, USA

^1aColleage of Chemistry and Chemical Engineering, Huanggang Normal University, Hubei, 438000, China

²Institute of Biomedical Science, National Chung-Hsing University, Taichung, Taiwan

³Transplant Medicine & Surgery Research Centre, Changhua Christian Hospital, Changhua, Taiwan

⁴Miyauchi Citrus Research Center, Shigoka-Machi, Takasaki, Gunma, 370-0845, Japan

⁵Graduate Institutes of Immunology, College of Medicine, National Taiwan University, Taipei 100, Taiwan

⁶Department of Medical Research and Education, Taichung Veterans General Hospital, Taichung, Taiwan, Republic of China.

⁷Division of Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, Changhua, Taiwan

⁸Rong Hsing Research Center for Translational Medicine, National Chung Hsing University

*To whom correspondence should be addressed:

Dr. Chi-Chen Lin, Institute of Biomedical Sciences, College of Life Science, National Chung Hsing University, Taichung, Taiwan FAX: +886-4-23592705, E-Mail: lincc@dragon.nchu.edu.tw

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